Bio-rad(AbdSerotech) alamarBlue® 阿爾瑪藍(lán) 細(xì)胞增殖和毒性
簡要描述:
Bio-rad(AbdSerotech) alamarBlue® 阿爾瑪藍(lán) 細(xì)胞增殖和毒性檢測:阿爾瑪藍(lán)(Alamar Blue)是一種細(xì)胞增殖檢測試劑,對各種人和哺乳動物細(xì)胞、細(xì)菌和真菌提供一種快速和敏感的細(xì)胞增殖和毒性檢測方法。
產(chǎn)品時間:2017-04-14
alamarBlue® 阿爾瑪藍(lán)(細(xì)胞增殖及毒性檢測試劑)
基本描述:
阿爾瑪藍(lán)(Alamar Blue)是一種細(xì)胞增殖檢測試劑,對各種人和哺乳動物細(xì)胞、細(xì)菌和真菌提供一種快速和敏感的細(xì)胞增殖和毒性檢測方法。
阿爾瑪藍(lán)(Alamar Blue)是一種基于刃天青(resazurin)的檢測試劑。刃天青作為一種一種氧化還原(REDOX)指示劑,根據(jù)細(xì)胞代謝還原情況發(fā)生顏色變化。氧化態(tài)的刃天青呈紫藍(lán)色且基本無熒光,其還原態(tài)產(chǎn)物試鹵靈(resorufin)轉(zhuǎn)變?yōu)榉奂t色且高度熒光,產(chǎn)生的熒光強(qiáng)度與呼吸活細(xì)胞數(shù)量呈正比。通過檢測呼吸過程中氧化水平,阿爾瑪藍(lán)用作一種定量檢測細(xì)胞活力和毒性的直接指示劑。阿爾瑪藍(lán)的顏色變化可通過普通分光光度計檢測吸光度變化,檢測波長為570nm,參考波長為600nm;阿爾瑪藍(lán)的熒光變化可通過熒光光度計檢測,激發(fā)波長在530~560nm之間,發(fā)射波長為590nm。
檢測原理:(細(xì)胞增殖實驗 Cell proliferation assay))
※ 生長中細(xì)胞引起Alamar Blue的化學(xué)還原反應(yīng);
※ 持續(xù)性細(xì)胞生長維持還原環(huán)境,使得Alamar Blue呈紅色,發(fā)強(qiáng)熒光;
※ 生長受抑制維持氧化環(huán)境,使得Alamar Blue呈藍(lán)色,無熒光;
※ 使用基于熒光或吸光值檢測的儀器收集數(shù)據(jù);
※ 熒光檢測的激發(fā)波長為530~560nm之間,發(fā)射波長為590nm;
※ 吸光值檢測的波長為570nm,參考波長為600nm;
保存方法:
室溫12個月穩(wěn)定,2-8°C保存20個月穩(wěn)定;避光保存;
產(chǎn)品優(yōu)勢:
- 簡單:水溶性,只需添加和測量即可;
- 靈活:顯色和熒光檢測,懸浮細(xì)胞和貼壁細(xì)胞;
- 無毒:對細(xì)胞無毒,對使用者和環(huán)境也無毒;
- 可靠:大量引用用于細(xì)胞毒性和活力檢測;
- 規(guī)模化:簡單放大體系用于高通量分析
- 高靈敏:zui低可檢測到50個細(xì)胞
- 穩(wěn)定高:*緩沖配方使其能用于長期研究;
- 經(jīng)濟(jì):不需要細(xì)胞裂解,使細(xì)胞能繼續(xù)培養(yǎng)用于后續(xù)分析;
訂購信息:品牌保證,現(xiàn)貨供應(yīng),大量使用文獻(xiàn),國內(nèi)外上萬實驗室的認(rèn)可品質(zhì),咨詢,,:。
品牌 | 產(chǎn)品名稱 | 產(chǎn)品編號 | 規(guī)格 | 價格(元) | 備注 |
Bio-rad(AbdSerotech) | alamarBlue® 阿爾瑪藍(lán) | BUF012B | 5ml | 335(特惠價) | 分裝 |
Bio-rad(AbdSerotech) | alamarBlue® 阿爾瑪藍(lán) | BUF012B | 25ml | 1165(特惠價) | 分裝 |
Bio-rad(AbdSerotech) | alamarBlue® 阿爾瑪藍(lán) | BUF012B | 100ml | 咨詢 | 原裝 |
【注1】:按照96孔板,每孔100µl終體積來算,5ml alamarBlue®可做500個孔;25ml可做2500個孔。
【注2】:alamarBlue® Technical Datasheet
引用文獻(xiàn)(AbdSerotech alamarBlue®,43篇):
- Lewis, C.S. et al. (2010) Local Antibiotic Delivery with Bovine Cancellous Chips.
- Alsford, S. and Horn, D. (2011) Elongator Protein 3b Negatively Regulates Ribosomal DNA Transcription in African Trypanosomes.
- Crilly, A. et al. (2011) Phosphodiesterase 4 (PDE4) regulation of proinflammatory cytokine and chemokine release from rheumatoid synovial membrane.
- Paget, C. et al. (2011) Potential Role of Invariant NKT Cells in the Control of Pulmonary Inflammation and CD8+ T Cell Response during Acute Influenza A Virus H3N2 Pneumonia.
- Lakhkar, N. et al. (2011) Titanium and strontium-doped phosphate glasses as vehicles for strontium ion delivery to cells.
- Wilson, B.A. et al. (2011) High-throughput screen identifies novel inhibitors of cancer biomarker a-methylacyl coenzyme A racemase (AMACR/P504S).
- Lau, L.I. et al. (2011) The Effect of Photooxidative Stress and Inflammatory Cytokine on Complement Factor H Expression in Retinal Pigment Epithelial Cells.
- Arlian, B.M. and Tinker, J.K. (2011) Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A2/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice.
- Voloshin, T. et al. (2011) G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist.
- Rao, T.D. et al. (2011) Dual-Fluorescence Isogenic High-Content Screening for MUC16/CA125 Selective Agents.
- Uitdehaag, J.C. et al. (2011) Multidimensional Profiling of CSF1R Screening Hits and Inhibitors: Assessing Cellular Activity, Target Residence Time, and Selectivity in a Higher Throughput Way.
- Rzhepishevska, O. et al (2011) The antibacterial activity of ga3+ is influenced by ligand complexation as well as the bacterial carbon source.
- Xu, S. et al. (2011) Marek's disease virus type 1 microRNA miR-M3 suppresses cisplatin-induced apoptosis by targeting Smad2 of the transforming growth factor beta signal pathway.
- Ardakani, A.G. et al. (2014) Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model.
- Nakayama, G.R. et al. (1997) Assessment of the Alamar Blue assay for cellular growth and viability in vitro.
- Diril, M.K. et al. (2012) Cyclin-dependent kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration.
- Warrier, T. et al. (2012) Antigen 85C inhibition restricts Mycobacterium tuberculosis growth through disruption of cord factor biosynthesis.
- Dreidax, D. et al. (2013) Low p14ARF expression in neuroblastoma cells is associated with repressed histone mark status, and enforced expression induces growth arrest and apoptosis.
- Wang, H. et al. (2014) Enhanced osteoblast responses to poly ether ether ketone surface modified by water plasma immersion ion implantation.
- Park, K.H. et al. (2014) Expression of polysialylated neural cell adhesion molecules on adult stem cells after neuronal differentiation of inner ear spiral ganglion neurons.
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— —Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科學(xué)和生物技術(shù)領(lǐng)域研究的試劑、儀器和實驗室消耗品與實驗服務(wù)工作,主要從事細(xì)胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。 本公司秉承“以人為本,以誠為信、合同守信”的經(jīng)營理念。堅持"品質(zhì)保障"的原則為廣大客戶提供優(yōu)質(zhì)產(chǎn)品。
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